Blog
Educational material, comparison studies and publications on SPR and other related techniques for researchers.
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Preparing for Characterization: Expression and Purification
When proposing a research project, there’s a number of project steps that require advanced planning. Asking questions like, “do I need to express and purify any protein(s)?”, and accounting for this in your project proposal saves you time and unexpected capital expenditures. In this article, we’ll compare the pros and cons of vital project parameters […]
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Comparison Study of Label-Free Biosensors Using Commercially Relevant Binding Systems
Summary Binding kinetics of biotinylated CD16a and IgG were characterized by comparing OpenSPR™ with a standard SPR instrument and a commonly-used BLI instrument. KD values measured using the OpenSPR™, the standard SPR instrument and the commonly-used BLI instrument were found to be 74.7 nM, 48.7 nM and 226 nM, respectively. This study supports the equivalency […]
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Our Mission: Improving Human Life by Helping Scientists Succeed
How is your research changing the world? Over the past 5 years, we’ve had the opportunity to help over 500 researchers worldwide succeed in their area of study. As we worked alongside these innovative researchers, we asked them what they are passionate about and what they need to be successful. We got a lot of […]
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OpenSPR Binding Data Uncovers Virulence Mechanism of Streptococcus pyogenes
Virulence factors are tools employed by pathogenic organisms to utilize, evade, colonize, suppress or destroy normal immune responses in a host. An example of a virulence factor is lipid A, a component of endotoxins produced by gram-negative bacteria, such as E. coli. In the context of treating pathogenic infections, virulence factors are the primary targets […]
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Pros & Cons of Fluorescence-Based Binding Assays
As the availability of affordable technology increases, reviewers are constantly requesting binding data during the publishing process. However, measuring only affinity or obtaining simplistic yes/no binding data may not satisfy all the requirements you need to successfully publish your research. There are many techniques that dig deeper beneath the surface than just affinity, including fluorescence […]
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How SPR Fits in Your Interaction Research Workflow
Trends in Publications How do you measure success as an academic researcher? Although there are many paths that a scientific research project takes, the end goal remains the same; publishing your work. Our mission at Nicoya is to help researchers publish faster with high-quality quantitative binding data. As the availability of affordable technology increases, reviewers […]
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Advancing Neuromuscular Disease Research with OpenSPR Binding Data
The human nervous system is a complex collection of over 100 billion nerve cells that regulate countless processes within the body. Within the processes of the muscular system, nerve and muscle cells are united with the neuromuscular junction (NMJ), playing a vital role in the development of neuromuscular diseases. The NMJ is also responsible for […]
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OpenSPR Binding Data Advances Delivery Method of Photodynamic Cancer Therapeutics
Dr. Xiaofeng Liu recently published a paper using binding data generated from OpenSPR to reveal an effective affibody-based photosensitizer carrier used in Photodynamic Therapy (PDT), a less invasive alternative to cancer treatments. Read the full article to see how OpenSPR provided Dr. Xiaofeng Liu with the key binding data needed to advance PDT.
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OpenSPR-XT Binding Data Opens New Doors for Targeting of RNA Binding Proteins for Glioblastoma Therapeutics
Dr. Luis Penalva recently published a paper using binding data generated from the OpenSPR-XT’s automated SPR technology assessing the effectiveness of luteolin binding to RNA binding protein, Msi1. Read the full article to see how OpenSPR-XT provided Dr. Penalva with the key binding data needed for his latest discovery of an effective glioblastoma treatment.
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Discovery of a Gene Regulation Network in P. aeruginosa Biofilm Production using OpenSPR Binding Data
Dr. Tung T. Hoang recently published a paper using binding data generated from OpenSPR to quantify the formation of a stable complex between 2 co-regulators of biofilm production and their target DNA binding sequence. Read the full article to see how OpenSPR provided Dr. Hoang with the key binding data needed to authenticate a gene regulation network in P. aeruginosa biofilm production.