Lipid Interactions

Quantitatively characterize your interaction with binding kinetics & affinity data. 

Protein_Lipid_1

Protein-Lipid

Ligand: Liposomes formed with 2 different phospholipids, immobilized onto Liposome (LIP-1) Sensors

Analyte: Proprietary protein

Liposome 1

kon = 810 M-1s-1

koff = 5.53×10-4 s-1

KD = 682 nM

Liposome 2

kon = 1100 M-1s-1

koff = 9.24×10-4 s-1

KD = 841 nM

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Outcomes:

  • Determine kon and koff (kinetics) and KD (affinity) value
  • Yes/No Binding

Use Cases:

  • Expressed protein interactions
  • Extracted protein interactions
  • Membrane proteins
  • Protein-lipid interactions
  • Lipid chains
  • Vesicles & lipid bilayers

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Overview

OpenSPR is the world’s only benchtop surface plasmon resonance (SPR) instrument. It provides high quality, label-free interaction analysis for a fraction of the cost of existing solutions. Our unique nano-structured sensor surface uses localized SPR (LSPR) to deliver repeatable, highly sensitive kinetic data.

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