Application Notes

Rapid, automated capture screening of influenza antiviral targets using Nicoya Alto® digital surface plasmon resonance

Overview Rapid screening and kinetic characterization of medium sized libraries is commonly seen as one of the initial steps within the drug discovery process. Screening assays are used to quickly tes...

Regeneration of His-tagged Streptavidin on OpenSPR® NTA Sensors Sensors

Overview Capture coupling techniques are advantageous to investigate biomolecular interactions as they provide specificity for immobilization on the OpenSPR real-time, label-free biosensor platform. S...

Characterization of HuCAL Fab-MH binding kinetics on Alto™ digital surface plasmon resonance using a Twin-Strep-tag® capture system

Overview In this application note, Alto™ digital SPR was used to characterize HuCAL Fab-MH kinetics using the TwinStrep-tag® Capture Kit, demonstrating the compatibility of the Strep-TactinXT® as ...

Accelerating influenza antibody therapeutics with Alto™ digital microfluidic-powered epitope binning and kinetics analysis

Overview Annual formulation updates for influenza vaccines and related antibody therapies are required to preserve immune recognition against different influenza subtypes. Characterizing the binding k...

Label-free characterization of transmembrane proteins using Alto™ digital SPR

Overview Many human diseases are known to be associated with transmembrane proteins (TPs), making them ideal candidates for drug development. Multi-pass transmembrane proteins are not stable outside o...

Characterization of Alto™ Sample Preparation Precision and Accuracy Enabled by DMF

Overview Precise and accurate liquid handling is crucial to obtaining repeatable, high-quality data with any analytical instrument. This is especially true when preparing samples for surface plasmon r...

Epitope Characterization of Influenza Antiviral Targets Using Alto™

Overview Rapid changes in influenza antiviral target proteins due to antigenic drift result in cloaking of the influenza virus from the immune system of vaccinated hosts. Hence, annual formulation upd...

Poster: Characterization of Therapeutic Antibodies using Digital SPR

Overview Alto is the world’s first SPR instrument to integrate digital microfluidics (DMF) with nanotechnology-based biosensors. With simultaneous detection across 16-channels, Alto provides high-th...

Screening of Unknown Antibodies in Complex Media with Alto™ Digital SPR

Overview Screening is a common tool used to quickly test activity of a large number of chemical or biological compounds. Commonly seen as one of the first steps within the drug discovery process, scre...

Characterization of biosimilars derived from PlantForm’s vivoXPRESS® technology with Alto™ Digital SPR

Overview Biological drugs, or biologics, have transformed patient outcomes worldwide due to their ability to treat diseases with a higher degree of precision and specificity compared to small-molecule...