Application Notes Archive - Nicoya - Improving Human Life by Helping Scientists Succeed https://nicoyalife.com Publication quality binding kinetics & affinity data on your benchtop. Thu, 11 Jan 2024 20:11:03 +0000 en-US hourly 1 https://wordpress.org/?v=5.8.8 https://nicoyalife.com/wp-content/uploads/2019/02/Nicoya-Favicon-150x150.png Application Notes Archive - Nicoya - Improving Human Life by Helping Scientists Succeed https://nicoyalife.com 32 32 Rapid, automated capture screening of influenza antiviral targets using Nicoya Alto® digital surface plasmon resonance https://nicoyalife.com/notes/rapid-automated-capture-screening-of-influenza-antiviral-targets-using-nicoya-alto-digital-spr/ Mon, 27 Nov 2023 18:29:49 +0000 https://nicoyalife.com/?post_type=notes&p=21144 Overview Rapid screening and kinetic characterization of medium sized libraries is commonly seen as one of the initial steps within the drug discovery process. Screening assays are used to quickly test and select the best candidates that bind to or affect a desired target. Label-free surface plasmon resonance (SPR) is often used to conduct medium […]

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]]> Regeneration of His-tagged Streptavidin on OpenSPR® NTA Sensors Sensors https://nicoyalife.com/notes/regeneration-of-his-tagged-streptavidin-on-openspr-nta-sensors/ Mon, 27 Nov 2023 18:18:48 +0000 https://nicoyalife.com/?post_type=notes&p=21139 Overview Capture coupling techniques are advantageous to investigate biomolecular interactions as they provide specificity for immobilization on the OpenSPR real-time, label-free biosensor platform. Streptavidin sensors offer a streptavidin coated sensor surface which can capture biotin-tagged ligands with high affinity. However, the biotin-streptavidin interaction is irreversible, limiting researchers to one biotinylated ligand per sensor. In this […]

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]]> Characterization of HuCAL Fab-MH binding kinetics on Alto™ digital surface plasmon resonance using a Twin-Strep-tag® capture system https://nicoyalife.com/notes/characterization-of-hucal-fab-mh-binding-kinetics-on-alto-digital-surface-plasmon-resonance-using-a-twin-strep-tag-capture-system/ Mon, 27 Nov 2023 16:48:10 +0000 https://nicoyalife.com/?post_type=notes&p=21111 Overview In this application note, Alto™ digital SPR was used to characterize HuCAL Fab-MH kinetics using the TwinStrep-tag® Capture Kit, demonstrating the compatibility of the Strep-TactinXT® as a capture molecule on the Alto™ platform. The streptavidin-biotin interaction is frequently leveraged for purification and detection of biomolecules due to its high affinity and stability. However, the […]

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]]> Accelerating influenza antibody therapeutics with Alto™ digital microfluidic-powered epitope binning and kinetics analysis https://nicoyalife.com/notes/accelerating-influenza-antibody-therapeutics-with-alto-digital-microfluidic-powered-epitope-binning-and-kinetics-analysis/ Mon, 27 Nov 2023 16:28:10 +0000 https://nicoyalife.com/?post_type=notes&p=21130 Overview Annual formulation updates for influenza vaccines and related antibody therapies are required to preserve immune recognition against different influenza subtypes. Characterizing the binding kinetics and epitope diversity of various antibodies to influenza viral antigens is essential for treating and preventing potential outbreaks. In collaboration with Sino Biological, a global leader in recombinant technology, we […]

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]]> Label-free characterization of transmembrane proteins using Alto™ digital SPR https://nicoyalife.com/notes/label-free-characterization-of-transmembrane-proteins-using-alto-digital-spr/ Mon, 27 Nov 2023 16:10:21 +0000 https://nicoyalife.com/?post_type=notes&p=20765 Overview Many human diseases are known to be associated with transmembrane proteins (TPs), making them ideal candidates for drug development. Multi-pass transmembrane proteins are not stable outside of the cell membrane environment, and are therefore difficult to purify and to express in large quantities. This has resulted in the under-characterization of a critical group of […]

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]]> Characterization of Alto™ Sample Preparation Precision and Accuracy Enabled by DMF https://nicoyalife.com/notes/alto-sample-preparation-precision-and-accuracy-enabled-by-dmf/ Wed, 26 Jul 2023 14:39:03 +0000 https://nicoyalife.com/?post_type=notes&p=20745 Overview Precise and accurate liquid handling is crucial to obtaining repeatable, high-quality data with any analytical instrument. This is especially true when preparing samples for surface plasmon resonance (SPR) experiments, since the sample concentration is the only known variable when solving for kinetic constants. Without accurate and precise liquid handling, error rapidly creeps into concentration […]

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]]> Epitope Characterization of Influenza Antiviral Targets Using Alto™ https://nicoyalife.com/notes/epitope-characterization-of-influenza-antiviral-targets-using-alto/ Wed, 05 Jul 2023 21:03:48 +0000 https://nicoyalife.com/?post_type=notes&p=20618 Overview Rapid changes in influenza antiviral target proteins due to antigenic drift result in cloaking of the influenza virus from the immune system of vaccinated hosts. Hence, annual formulation updates for influenza vaccines and related antibody therapies are required to preserve immune recognition against different influenza subtypes. As such, characterizing the binding kinetics and epitope […]

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]]> Poster: Characterization of Therapeutic Antibodies using Digital SPR https://nicoyalife.com/notes/poster-characterization-of-therapeutic-antibodies-using-digital-spr/ Fri, 24 Feb 2023 17:19:51 +0000 https://nicoyalife.com/?post_type=notes&p=19833 Overview Alto is the world’s first SPR instrument to integrate digital microfluidics (DMF) with nanotechnology-based biosensors. With simultaneous detection across 16-channels, Alto provides high-throughput analysis of up to 48 unique targets, while further streamlining user workflows with automated sample dilutions, disposable fluidics, and sample requirements reduced by up to 200X. In collaboration with Sino Biological, […]

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]]> Screening of Unknown Antibodies in Complex Media with Alto™ Digital SPR https://nicoyalife.com/notes/screening-of-unknown-antibodies-in-complex-media-using-alto/ Wed, 15 Feb 2023 21:17:17 +0000 https://nicoyalife.com/?post_type=notes&p=19820 Overview Screening is a common tool used to quickly test activity of a large number of chemical or biological compounds. Commonly seen as one of the first steps within the drug discovery process, screening assays are used on large scale libraries to quickly test and select candidates that bind to or affect a desired target. […]

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]]> Characterization of biosimilars derived from PlantForm’s vivoXPRESS® technology with Alto™ Digital SPR https://nicoyalife.com/notes/characterization_of_plantform_vivoxpress_biosimilars_with_alto/ Wed, 08 Feb 2023 18:32:01 +0000 https://nicoyalife.com/?post_type=notes&p=19809 Overview Biological drugs, or biologics, have transformed patient outcomes worldwide due to their ability to treat diseases with a higher degree of precision and specificity compared to small-molecule drugs. While biosimilars, which are highly similar copies of existing biologics, have increased affordability to biologics due to their abbreviated approval process, there still remains a need […]

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